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Stable Isotope Geochemistry

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Subject:
Re: Mice Blood
From:
Marilyn Fogel <[log in to unmask]>
Reply To:
[log in to unmask]
Date:
Wed, 11 Jul 2007 21:53:27 -0400
Content-Type:
text/plain
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You need to think about what is in freeze-dried blood--the red and white
blood cells and also the proteins and amino acids, sugars etc. dissolved
in blood. The water will be gone.

If you want the water, you can spin down the cells, pipet off the plasma.
Keep in mind, though, that even plasma has organic molecules in it that
will affect both O and H isotope values. You other choice is to distill
the water off the blood.

Vampire

> Dear List Members,
>
> A colleague of mine is interested in determining H and O values from mice
> blood.  I was thinking that O could be done by pyrolysis from freeze-dried
> blood.  How about hydrogen...any reason that the water H in blood would
> not
> equilibrate with head gas and be able to be determined in a gas bench?
> Any
> advice on either idea would be appreciated.
>
> Dave Mrofka
> Earth Sciences
> UC Riverside
>
>
>


Dr. Marilyn L. Fogel
Geophysical Laboratory
Carnegie Institution of Washington
5251 Broad Branch Rd., NW
Washington, DC 20015 USA
Phone: 202-478-8981
FAX: 202-478-8901
Website: http://fogel.ciw.edu

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