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Subject:

Re: Pseudomonas chlororaphis

From:

Andrew Schauer <[log in to unmask]>

Reply To:

Stable Isotope Geochemistry <[log in to unmask]>

Date:

Wed, 23 Apr 2008 08:33:53 -0700

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Hi Helena,

Pseudomonas chlororaphis and Pseudomonas aureofaciens are the two used for the denitrifier work. aureofaciens , due to its differing enzyme metal center, exchanges less oxygen than the chlororaphis strain. both work for 15N. 

Stigman et al. 2001. Anal. Chem. 73:4145-4153.

Last I checked, aureofaciens was available from ATCC. 

andy


 
Andrew Schauer
ISOLAB
Department of Earth and Space Sciences
University of Washington
Seattle, WA 98195

206.543.6327
[log in to unmask]

----- Original Message ----
From: Helena JÃ¤ntti <[log in to unmask]>
To: [log in to unmask]
Sent: Wednesday, April 23, 2008 2:01:08 AM
Subject: [ISOGEOCHEM] Pseudomonas chlororaphis

Does anyone where I could get the bacterial strain of Pseudomonas 
chlororaphis that lacks the N2O reductase capacity? The ATCC # is 43928 
but is not currently available from ATCC. Alternatively, does anyone 
know are there any other strains that could be used in the 
"denitrifier"-method that is used in 15NO3 analyzes?

Best regards,

Helena Jäntti


Helena Jäntti (Msc)
Researcher
University of Helsinki/Division of Aquatic Sciences

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