Hi Roland:
Yes.  It is a straightforward assay.  If you have a good spectrophotometer
you can follow the reduction reaction of NAD to NADH at 340 nm wavelength.
 The only question is whether you want to do this assay in crude extracts
or in a partially purified protein preparation and also whether you want
to analyze the isotopic signature of the CO2 generated.  You can send me
an e-mail to the below given e-mail address so that I can certainly help
you with whatever I know in this regard.

Best Regards

Madhavan

Dr. S.Madhavan
Asst. Director, UNL Honors Program
Department of Biochemistry
 N 251 Beadle Center
University of Nebraska-Lincoln
NE 68588-0664, USA
Ph: 402-472-9802
Fax: 402-472-7842
E-mail: [log in to unmask]





On 5/31/17, 8:21 AM, "Stable Isotope Geochemistry on behalf of Wolfram
Meier-Augenstein (pals)" <[log in to unmask] on behalf of
[log in to unmask]> wrote:

>Hi Roland,
>
>
>A former colleague of mine should be able to help you with this
>straightforward assay.
>
>Dr. Robert Hancock,
>Cell & Molecular Sciences,
>The James Hutton Institute,
>Invergowrie,
>Dundee DD2 5DA
>
>Tel: +44(0)1382 568 779
>e-mail: [log in to unmask]
>
>
>Best,
>
>Wolfram
>
>
>
>-----Original Message-----
>From: Stable Isotope Geochemistry [mailto:[log in to unmask]] On
>Behalf Of Roland A. Werner
>Sent: 31 May 2017 12:29
>To: [log in to unmask]
>Subject: [ISOGEOCHEM] measurement of enzyme activity
>
>Dear Isotope community,
>
>we are looking for a laboratory with knowledge on measurement of enzyme
>activities in plant material. In special, we are interested in malic
>enzyme (EC 1.1.1.39) catalysing the reaction malate + NAD+  <=> pyruvate
>+ CO2 + NADH.
>Is there someone who is doing that sort of analysis on routine base?
>Many thanks in advance
>Best regards
>
>Roland
>
>--
>####################################
>
>Dr. Roland A. Werner
>Institut fuer Agrarwissenschaften
>Massenspektrometer-Labor
>ETH Zuerich, LFW C48.1
>Universitaetsstrasse 2
>CH-8092 Zuerich
>Schweiz
>
>Tel. Buero:  ++41/(0)44/632 6754
>
>Tel. IsoLab: ++41/(0)44/632 6694
>
>No Fax device
>
>e-mail: [log in to unmask]
>
>URL:    Personal ETH website
>         http://www.gl.ethz.ch/people/person-detail.html?persid=116463
>         Researchgate Account
>         http://www.researchgate.net/profile/Roland_Werner/
>         Academia.edu Account
>         https://independent.academia.edu/RolandWerner
>
>
>####################################
>
>----------
>
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