Dom Adair

Try Ian Begley, Scottish Crop Research Institute, Dundee.  He has a
continuous flow set up and measure H/D and 18O in beas.

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Phone 01382 562731.

Hope this helps,

Paul Brooks.




At 03:32 PM 9/23/98 +0100, you wrote:
>Hi.
>
>I'm a PhD student in the Zoology Department at Cambridge researching
>energy expenditure in bees. I use doubly-labelled water to calculate
>energy turnover and thus generate microlitre volumes (typically in 5
>microlitre aliquots) of labelled body water. I have a small vacuum rig for
>freeze drying the bees but it's not really adequate to use for further
>reduction of the DLW samples as preparation for IRMS analysis.
>
>Does anyone know of a laboratory that might be able to analyse my
>samples? They total about 100 bees (about 400 aliquots of DLW) and the
>isotope concentration is likely to be high (initial concentrations
>estimated to range from about 300 - 700 ppm (some samples) and
>others less (turned over). There are also some samples with a very high
>concentration (10% APE with various amounts of fractionation).
>I was hoping that they could be analysed using continous-flow pyrolysis
>IRMS as this would dispense with the need for separate reduction and
hopefully
>cope with the small volumes. Does anyone out there do this? Or know of a
>good alternative that would suit my needs?
>
>Any help much appreciated.
>
>Cheers,
>Dom Adair.
>
>Insect Flight Group,
>Department of Zoology,
>Downing Street,
>Cambridge CB2 3EJ
>Tel. 01223 334478
>
>