Lars,

we frequently silanize our glass tubes. But the reagent is MSTFA/CH3CN.
MSTFA is usually used to derivatize non-volatile analytes to prepare them
for GC-analysis. MSTFA does not give any acid products and thus might be
well suited for your needs.

What does the SPE procedure exactly look like? I have no idea about "NH2"
in that context.

Best ...

        Uli Flenker
        Institute of Biochemistry
        German Sports University Cologne
        Carl-Diem-Weg 6

        50933 Cologne / Germany

        Phone 0049/0221/4982-493


On Wed, 27 Sep 2000, Lars Löfgren wrote:

> Dear listmember,
> I am looking for improved recovery (from 95% to >99%) by less adsorption of
> lipids (free fatty acids) from liquid liquid extraction and look for
> silanized glass tubes (about 10 ml volume). Does anyone know of a supplier?
>
> Did anybody else found some difficulties getting home made tubes 100% free
> of acidic compounds? They (dichlordimetyhylsilane and its reaction products)
> tend to remain on the surface and ruin my NH2 SPE separation of NEFA from
> neutral lipids after evaporation to dryness.
>
> Lars
>