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Stable Isotope Geochemistry

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Stable Isotope Geochemistry <[log in to unmask]>
Subject:
enrichment question (calculation)
From:
Todd Miller <[log in to unmask]>
Date:
Sun, 14 Apr 2002 16:53:26 -0700
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Stable Isotope Geochemistry <[log in to unmask]>
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Greetings,

I am about to enrich fish food for Pacific herring I am holding in
captivity and I have a rather simple question regarding my enrichment
procedure.  I plan to enrich food with a 15N-enriched (min. 98+ atom
percent) essential amino acid (L-Phenylalanine).  How do I determine
the grams of actual 15N in, say, 5 grams of 98+ atom percent 15N?
Since L-Phenylalanine is 8.48%N, then if I have 5g of 98+atom percent
15N I should have 0.424g of 15N.  Yes?  No?  Any input on this would be
greatly appreciated.

Also, anyone out there doing similar work (vertebrates and inverts)?  I
would like to hear from you since this aspect of natural enrichment
(i.e. controlled experiments) is something I would like to discuss with
others'.  Thanks.

Todd

Todd W. Miller
Dept. Fisheries and Wildlife
Oregon State University
Hatfield Marine Science Center
2030 South Marine Science Dr.
Newport, Oregon  97365 USA
ph 541-867-0397
fax 541-867-0138
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