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Stable Isotope Geochemistry

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Subject:
Re: Isotopic analysis of cellulose
From:
Eric Lichtfouse <[log in to unmask]>
Reply To:
Stable Isotope Geochemistry <[log in to unmask]>
Date:
Thu, 8 Oct 1998 05:51:47 +0100
Content-Type:
text/plain
Parts/Attachments:
text/plain (17 lines)
>(2) Is anybody aware of an alternative to the benzene-methanol
>extraction for the preparation of cellulose extracts. I'm not too happy
>about the benzene (unless there's no alternative).

15 min. sonication under a ventilated hood of finely grounded plant debris
into CHCl3 MeOH (3/1 v/v) then filtration (3-4 times) works well to remove
lipids and resins.

Dr Eric LICHTFOUSE
Laboratoire Sols et Environnement
INRA-ENSAIA/INPL, BP 172
2, avenue de la foret de Haye
F-54505 Vandoeuvre-les-Nancy Cedex, France
Phone (33) 3 83 59 58 99 (-58 45, -58 65)
Fax (33) 3 83 59 57 91
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