I realise this has probably been discussed several times before, but here
We are about to analyse carbon and nitrogen SIRs in some small(ish) marine
crustacea (certainly too small to dissect out muscle tissue in most instances).
I've noticed that in some of the lit. these types of sample are acid washed
to remove inorganic carbonates, which seems sensible if the inorganic
carbon component of the cuticle is high (I can't track down anything that
gives the % inorganic C component of this but it is probably very variable
anyway). However in some studies the issue is completely ignored and whole
animals are just homogenised for analysis.
Anyone any idea why this is?
What are current thoughts and does anyone have any particular protocols for
I know that some people have found that extended periods in HCl may alter
d15N (thanks Susan), so at the moment I am thinking of splitting each
sample acid washing one fraction for C and using the remainder for N?
Your thoughts would be greatly appreciated
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