I have some questions (due to lack of manuals/help options of the software and/or
insufficient explanation in the help menues) regarding the two software packages we
use with an Isochrom (software EA 1.41) and an IsoPrime (Masslynx 4.0) .
Can I create a printout or file of the raw data of a run and if so, does it give the values
with or without background substraction or both (on the basis that you ticked
background substraction in the method file)?
I guess this is also a question about what exactly "raw data" means, i.e. is it the
areas/ratios the machine measures without any corrections applied?
Also in this context: can you recalculate values without background substraction after a
run has finished, although the background substraction was ticked in the method file?
What does the zeroing do exactly?
If I go on results, raw data, (after loading a data file in the Isodata software) nothing
happens although the software asks me whether I would like to print the raw data. Is
that a common problem or just with our software?
Can you get a batch data file from a whole run where all data are included (ratios,
deltas of the ref gas and the sample, areas, amplitudes etc.)?
Where in the run is the background measured, if you have ticked "left limit" and not
"average" in the background parameters? Is that at the very start of the run or left of the
sample peak, i.e. just before HS is closed?
If you go into species parameters, sample peak, you can chose whether you give the
software a window where to look for the sample peak or allow the full range. If I chose a
window, the software tells me that it is unable to "find 463 possible background points
(no peak) in 647 scans reducing required minimum to 281 points". Is this bad, i.e. does
that give you a bad background measurement?
Where can you set the detection limit of a peak to a certain amplitude (so that the
nitrogen peak is not detected)?
On the same window you can change the baseline detection parameters. I am not sure
what the different options exactly do:
peak start/end (does it just measure before and after the sample peak and calculates
mean or forces horizontal)?
Why does the message "warning, beam zero cuts peak 1 intensity" come up although I
have not chosen beam zero and if so "allow base line to cut intensity" is unticked?
Thanks a lot for your help!! I'm sure this is not everything I was wondering about but
that'll do for now :-)
Environmental Biology Group
Research School of Biological Sciences
Australian National University
Canberra, ACT, Australia
email: [log in to unmask]