Quick question regarding the floating changeover that sits in the oven on
the new Trace GC and IsoLink system... This changeover (for lack of a
better word) has a T-interface, input comes from the column, one output
goes to the oxidation/reduction reactor, and the other goes to the
pyrolysis reactor... So in essence your sample is being split post-column
into two aliquots, even though you can only run one isotope system at a
time... This is nice (fancy German engineering) because it means you can
run compound-specific C, N, and H from the same sample in the same run,
but it decreases peak intensity/injection amount ratios...
Has anyone capped on the output on this T-interface to increase
sensitivity? Right now we are only interested in running compound-specific
N isotopes and I would rather have greater sensitivity than the ability to
run compound-specific H from the same sample in the same run... So I would
like to cap the output that goes to the hydrolysis column to ensure that
all of our separated aminos go to the oxidation/reduction reactor...
Any insights would be appreciated...
Seth D. Newsome, Ph.D.
University of Wyoming
Department of Zoology and Physiology
1000 East University Avenue, Dept. 3166
Laramie, WY 82071
Email: [log in to unmask]