Hi Fred, in addition to Wolfram's papers, there's a couple of other
papers that have looked at peak size, combustion conditions, and
background. The effects of peak shape (tailing/fronting), to my
knowledge, has not been systematically studied for a given amount of
material on column (say, by changing phase ratio). My experience has
been that overloading doesn't compromise d13C linearity so long as
baseline resolution is still achieved . . . which is a big "if". Most
of the work is on d13C, not D/H.
Goodman and Brenna, J Chrom A 1995 (discussion of peak size)
Merritt and Hayes, 1994 An. Chem
Merritt and Hayes, 1995 An Chem (discusses effects of incomplete
oxidation, among other things)
Wolfram's 1996 J Chrom A paper and his other refs.
Sacks, Zhang, and Brenna, 2007, An Chem (buried in the discussion is a
section on the role of peak width and background noise in compromising
As I've said in other posts, reproducibly defining the background is
usually the main issue with getting good precision.
> Dear IsoGeochem
> I have very difficult to find literature that concerns the subject of
> the quality of the chromatography and how it affects the stable
> isotope ratios in GC-IRMS.
> Iím thinking especially about peak shape (tailing/fronting, overload,
> poor oxidation/reduction of oven, leakages, bad column etc), and not
> so much about poor separation.
> Has anyone on this list done studies into this subject, that could
> tell me a little about it, or maybe show some data on it? How does
> peak shape, background and signal strength correlate and affect the
> isotope ratios?
> Thank you very much!
> Best regards,
> Fred Martin Kaaby
Assistant Professor of Enology
Dept. of Food Science & Technology
NYS Ag. Experiment Station
Geneva, NY 14456