I am using 1.2 microliter Hamilton syringes (part no. 203185/01) and
they work well if you use a slow sample fill speed, sample volume less
than 1 microliter, and also clean them as often as possible as
sometimes you don't know how well the natural water samples were
filtered or what they contain. Cheers. Mihai Lefticariu (Southern
Illinois University Carbondale)
On Fri, Jan 11, 2013 at 2:49 PM, Laura Rosales Lagarde
<[log in to unmask]> wrote:
> Thank you Ben, Jim, Paul, Gerry for your quick and useful response.
> The "hidden"bungee cord to the right and back of the autosampler was pretty
> stretched out so that was likely the source of the problems with the post-
> cleaning. We changed earlier the one to the left which was also in bad
> shape. Matt Lachniet changed the cord for some tent poles bungee cord and
> the problem seems to have snapped out, so far. To take the back plate out
> you need to access three torx screws on each side, so the needle guide needs
> to go pretty far down.
> We also reset the definition of the tray, the wash, and the injection port.
> They were off for a little bit.
> Hopefully fixing the bungee cords and resetting the definition in the
> autosampler will increase the reproducibility of the peaks so we can get
> better precision in the analysis. The other weak link in the system are the
> syringes. The ones that seem to work best so far are 0.5 microliter SGE
> syringes (P/N 445, 0.5 BR-S/0.63). Have you found others that work better?
> Take care,
> Laura Rosales-Lagarde
> Post-doc Scholar
> UNLV Geoscience Department
> 4505 S. Maryland Parkway
> Las Vegas NV 89154-4022
> SEB 3106 Tel. (702)-895-3595
> [log in to unmask], [log in to unmask]