Are all of the hydrogens in the molecule deuterium? If so I don't think this is a chromatography issue.
I am not sure how the collector will handle a completely deuterated compound. Perhaps others will chime in.
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> On May 20, 2016, at 7:56 AM, Isabelle Chery <[log in to unmask]> wrote:
> Hello all,
> I would like to analyse a uniformly labelled fatty acid with Deuterium (D31 Palmitc Acid) after derivatization to be volatile as methyl ester by Gc pyrolyse IRMS.
> I use a Zebron ZB Wax capillary GC column to separate different Fames (C14:0 to C22:0). The injector is at 220°C and the high temperature conversion reactor is at 1420°C.
> The D31-Palmitic acid is totally separated from the natural Palmitic acid by GC.
> I have beautiful peaks for natural abundance of FAMEs (mass 2 and mass 3) but for the D31-Palmitc acid, the peak shape (only mass 3 is present) is trailing and is not symmetric.
> I have changed the insert, the syringe, cut the column, and put a new reactor tube. But the problem is always being.
> Does anyone could help me?