As Geert has pointed out already, choice of polarity of stationary phase depends on choice of derivatisation.
If you go for an apolar derivatisation (e.g silylation) an apolar stationary phase like a DB-5 would work well. That being said, there are some very good reasons not to opt for silalation in the context of GC-IRMS.
If you go for a mid-polar derivatisation like NAP or ethylformates a mid-polar stationary phase is the column of choice. Irrespective of your final choice being a DB-1705 or a DB-35, be sure to go for the crosslinked if there is a choice between cross-linked and not-cross-linked.
Also note, mid-polar (as well as polar) columns are light and oxygen sensitive. When not in use store the column/s in their box and seal the ends.
Prof. Dr W Meier-Augenstein, CChem, FRSC
Stable Isotope Forensics & Analytical Sciences
Robert Gordon University
School of Pharmacy and Life Sciences
The Ian Wood Building
E-mail: [log in to unmask]
From: Stable Isotope Geochemistry <[log in to unmask]> on behalf of Dana Biasatti <[log in to unmask]>
Sent: 02 February 2017 19:52:24
To: [log in to unmask]
Subject: [ISOGEOCHEM] Opinion on GC column for running amino acids?
I was just wondering if those of you that have experience running amino acids on a GC/IRMS could share your column preference. We are trying to decide between a DB-5, DB-35, and a VF-23MS. Thank you very much in advance for your response!!!
Stable Isotope Specialist
Center for Environmental Science and Technology
University of Notre Dame
Notre Dame, IN
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