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MEDLIB-L  January 1996, Week 2

MEDLIB-L January 1996, Week 2

Subject:

Micropipette Positioning for Patch Clamp.

From:

Ulrich Rexhausen <[log in to unmask]>

Reply-To:

Medical Libraries Discussion List <[log in to unmask]>

Date:

Wed, 10 Jan 1996 19:10:28 GMT

Content-Type:

text/plain

Parts/Attachments:

Parts/Attachments

text/plain (65 lines)

Hallo,
Iam working at a B.Sc Thesis in "Sensorical Biophysics" about AUTOMATICAL
PRECISION LASER POSITIONING OF MICROPIPETTES FOR PATCH CLAMP SETUPS in
Elektrophysiology. I would be happy if there are people who are interested
and have experience or a good idea about that. Maybe there is someone
working on the same thing, because all "Patch Clampers" and other people
dealing with micropositioning should be confrontated with the same
problem.
 
First I explain the technique and then the idea for a Micropipette
Positioning System:
 
The Patch Clamp technique is an important cell physiological method that
allows the properties and dynamics of single ion channels in the membrane
of biological cells to be studied (in 1991 Sakman and Neher won the Nobel
Price in medicine for the development of this technique).
 
Since the pipette tip is fragile optical methods for the positioning are
favorized.The problem is, that the length of the pipettes vary. This makes
the positioning of the pipettes more complicated. Because of that it is
important to determine the position of the pipette tip as a fixed
reference point, so that it is possible to position the pipette
automatically by use of the manipulator in front of the cell. The idea to
get a fixed reference point for the tip of the pipette is:
 
                             //
                            //<== pipette
                //
               lens       //
_______          |       //          ||
       |         | o    //         o ||
       | ------- |    o /       o    ||
       | ------- |       o   o       ||
laser  | ------- |         x         || <== array of Photodetectors
       | ------- |       o   o       ||
       | ------- |    o    |    o    ||
_______|         | o       |       o ||
                 |         |         ||
                |
                           | laser fokus
 
 
 
A diode laser, a lens to focus its beam, and a circular array of
photodetectors are fixed to the microscope stage. The micropipette inside
the focused laser beam produces a characteristic shadowing which can be
detected by the array of photodetectors. Quantifyinig the distortion of
the illumination of the detector array as a function of x,y, and z enables
the position of the pipette tip to the focus of the laser beam, which is
the reference point.
 
 
Another method could be the use of a CCD-camera. The pipette is imaged on
a chip and the darkening of the pixels at the chip are read by means of
computer.
 
Now you know all thoughts I have aboute this. So if you are interessted or
experienced in AUTOMATICAL PRESICION LASER POSITIONING OF MICROPIPETTES it
would be great to hear from you.
 
 
yours Detlef Repplinger
 
(email : [log in to unmask])

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November 2002, Week 1
October 2002, Week 5
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September 2002, Week 5
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September 2002, Week 1
August 2002, Week 5
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August 2002, Week 1
July 2002, Week 5
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July 2002, Week 1
June 2002, Week 5
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June 2002, Week 1
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April 2002, Week 5
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April 2002, Week 3
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April 2002, Week 1
March 2002, Week 5
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February 2002, Week 1
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January 2002, Week 3
January 2002, Week 2
January 2002, Week 1
December 2001, Week 5
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December 2001, Week 3
December 2001, Week 2
December 2001, Week 1
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November 2001, Week 1
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April 2001, Week 3
April 2001, Week 2
April 2001, Week 1
March 2001, Week 5
March 2001, Week 4
March 2001, Week 3
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March 2001, Week 1
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February 2001, Week 3
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February 2001, Week 1
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January 2001, Week 1
December 2000, Week 5
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December 2000, Week 3
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December 2000, Week 1
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November 2000, Week 1
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October 2000, Week 1
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February 2000, Week 3
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February 2000, Week 1
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January 2000, Week 3
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January 2000, Week 1
December 1999, Week 5
December 1999, Week 4
December 1999, Week 3
December 1999, Week 2
December 1999, Week 1
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January 1999, Week 3
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December 1998, Week 5
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December 1998, Week 3
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December 1998, Week 1
November 1998, Week 5
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October 1998, Week 5
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October 1998, Week 1
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September 1998, Week 1
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April 1998, Week 5
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April 1998, Week 3
April 1998, Week 2
April 1998, Week 1
March 1998, Week 5
March 1998, Week 4
March 1998, Week 3
March 1998, Week 2
March 1998, Week 1
February 1998, Week 4
February 1998, Week 3
February 1998, Week 2
February 1998, Week 1
January 1998, Week 5
January 1998, Week 4
January 1998, Week 3
January 1998, Week 2
January 1998, Week 1
December 1997, Week 5
December 1997, Week 4
December 1997, Week 3
December 1997, Week 2
December 1997, Week 1
November 1997, Week 5
November 1997, Week 4
November 1997, Week 3
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November 1997, Week 1
October 1997, Week 5
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October 1997, Week 3
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October 1997, Week 1
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April 1997, Week 5
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April 1997, Week 1
March 1997, Week 5
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January 1997, Week 5
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January 1997, Week 1
December 1996, Week 5
December 1996, Week 4
December 1996, Week 3
December 1996, Week 2
December 1996, Week 1
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November 1996, Week 3
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November 1996, Week 1
October 1996, Week 5
October 1996, Week 4
October 1996, Week 3
October 1996, Week 2
October 1996, Week 1
September 1996, Week 5
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April 1996, Week 1
March 1996, Week 5
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March 1996, Week 2
March 1996, Week 1
February 1996, Week 5
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February 1996, Week 2
February 1996, Week 1
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January 1996, Week 1
December 1995, Week 5
December 1995, Week 4
December 1995, Week 3
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July 1995, Week 2
July 1995, Week 1
June 1995, Week 5
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April 1994, Week 5
April 1994, Week 4
April 1994, Week 3
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