Of course it is. How you go about it only depends on the answers you
are interested in and on the typ of IRMS system at your disposal.
In principle you have two choices: (1) separation of the individual
AA from your protein hydrolysate by means of either PAGE or HPLC
(underivatised) or a Prep. GC (derivatised) and subsequent
measurement of the isolated AA on a CF-IRMS coupled to an elemental
analyser. (2) Derivatisation of the AA mix from your purified protein
hydrolysate and subsequent measurement by means of GC-C-IRMS.
Mike Rennies' group (to which I belong) has got some experience in
both methods. If you should be interested in method (1), please, get
in touch with Ayyub Patel ([log in to unmask]). If,
however, you wish to learn more about method, please, do not hesitate
to contact me.
Dr. W. Meier-Augenstein
Hon. Lecturer in Chemistry
University of Dundee
Dept. of Anatomy & Physiology
DUNDEE DD1 4HN
Tel.: +44-(0)1382-34/5124, /4968
e-mail: [log in to unmask]
or : [log in to unmask]