Dear colleagues - I am a relative novice to the field of SI applications to aquatic ecology studies, and I'm gearing up for several pilot studies involving Great Lakes trophic transfer problems. I've read much, and have a freezer full of both archived and unprocessed fish at my disposal. (collaborations welcome!) Does anyone have a detailed protocol that they follow / highly recommend for the preparation of fish tissue (be it whole ground fish or specific organ/muscle) for stable isotope (C,N) studies? If it is (they are) published, could I please have the citation(s)? Many thanks for your help. **************************************************************************** * Dr. James P. Hickey -- Section Leader, Ecosystem Health * USGS/Great Lakes Science Center * Ann Arbor, MI 48105 * T: 734-214-7233; F: 734-994-8780 [log in to unmask] ***************************************************************************