Alex, I wouldn't be concerned with C exchange between methane and CO2. But I will be weary of the different sources of CO2 in breath (i.e., rumen fermentation and the host). If you are interested in the animal I would measure blood CO2, if you interest is in the rumen, I would take samples directly from the dorsal sac. Regards Juan At 03:09 PM 4/8/2005, you wrote: >Hi everyone, > >As anyone ever heard of a possible contamination of 13CO2 values in cows >and steers breath samples due to isotopic exchange between CO2 and methane >? If this is occuring, how fast could it affect carbon values ? > >Thank you ! > >Alex > >Alexandre Myre, Research Officer, University Laval.