I was about to say the same. Bad idea. The HCl will cause damage to your freeze dryer, and particularly the vacuum pump (unless you put at least 2 liquid N2 traps in the line). As you know, HCl itself is a gas (bp -85 C at 1013 mbar) and concentrated (or fuming) HCl is a 38% solution of HCl in water. Your 6 molar HCl is roughly 2/3 of that. Under vacuum, virtually the first thing to be pumped off will be gaseous HCl. Wolfram > -----Original Message----- > From: Stable Isotope Geochemistry > [mailto:[log in to unmask]] On Behalf Of Stephanie Ewing > Sent: 06 July 2005 22:41 > To: [log in to unmask] > Subject: Re: siderite removal from clay/organics for d13C > > > The HCl will be hard on your freeze dryer unless > you have an effective trap. There's been lots of > discussion of various alternate acids on this > list. I've used 100% phosphoric, which has the > benefit of low vapor pressure, but makes > subsequent dehydration challenging. > > Stephanie > > >Folks, > > > >We're trying to separate siderite from soil/clay samples so > that we may > >analyze the organic components of the soil for d13C, d15N and CN > >ratios. The refs I've come across focus on 'carbonate' removal and > >indicate that siderite is less soluble. I found I can > dissolve siderite > >in 6 molar HCl at 50°C overnite, or soaking in 6 molar HCl > at 25°C for > >several days. To remove excess HCl I plan to freeze dry the > samples and > >not pour out any of the acid in case some of the organics have also > >gone into solution (I think this is the same idea as vapour removal). > > > >Any comments or suggestions regarding possible effects others have > >observed using this method, or better methods before I follow the > >tedious path of sample preparation testing? The first line > of testing I > >was going to do was to dose IAEA-C5 (wood) with siderite and see if > >THIS pretreatment affected its d13C values. > > > >Thanks in advance for saving me time, > > > >Toti. > > > > > >Toti Larson Ph.D. > >Los Alamos National Laboratory > >Earth and Environmental Sciences 505.667.9894 (lab) > >Los Alamos, New Mexico 87545 550.667.8006 (office) > > > -- > *** > Ecosystem Sciences Division > Department of Environmental Science, Policy & Management > Office: 45 Hilgard Hall > Mail to: 137 Mulford Hall #3114 > University of California > Berkeley, CA 94720-3114 > tel. 510-643-6910 > fax 510-643-5098 >