We soak the Nafion in methanol. Interesting to hear that acetone can make it too soft... not a problem with methanol. By the way, don't use ethanol - mass of 46!!! That will leave you with a headache. Select Nafion tubing TT-020, which when swollen with ethanol (let soak for a minute) will slip over 0.5mm OD capillary tubing then dry to a tight fit. Paul Eby University of Victoria At 01:02 AM 21/11/2005, you wrote: >Hi Karyne, >Spit only works if it is at the correct pH and consistency. You >need to make sure that your diet contains plenty of glutinous >material for a week prior to sealing the nafion. I have found that >a diet of porridge, pine cones and treacle toffee works best. Be >careful that you don't overdo the treacle toffee however,otherwise >the spit will solidify on contact with air and will not remain >liquid long enough to transfer to the nafion/fused silica joint to >ensure a leak-tight seal. >Hope this helps, >Steve > >P.S. >Seriously, I have no idea where the spit thing came from. Normally >just dip the very end of the fused silica into some acetone and >carefully insert into the nafion tube by 1-2cm and leave to >evaporate. (The nafion tube will become too soft and collapse if too >much acetone is used). If the nafion tube is too wide, try using >two short lengths of the next size fused silica and when sealed >couple up to these with the 320um. >Or: using 320um throughout with 030 nafion, insert the 320 by 2cm >into the nafion and use a very small amount of something like >Araldite to seal the joint. If using this method carefully move the >fused silica backwards and forwards by a few mm just to ensure the >Araldite fills the joint, then leave to dry. >Alternatively, use 020 nafion with 320um fused silica. This is a >tight fit but works well if you are careful. >Steve > > >>From: Karyne Rogers <[log in to unmask]> >>Reply-To: Stable Isotope Geochemistry <[log in to unmask]> >>To: [log in to unmask] >>Subject: [ISOGEOCHEM] nafion tubing...what's your trick? >>Date: Mon, 21 Nov 2005 14:25:28 +1300 >> >>Hi Tim >> >>When Steve Welsh from Sercon was out to install the GC, he showed me how >>to install the nafion, I've never had to do it since. I remember he said >>just spit on your fingers etc, and the spit will act as glue and shrink >>the nafion onto the fused silica. Well, I've tried spit, methanol, >>ethanol, but nothing works, the tubing still slides off the fused silica >>very easily. I'm using the nafion that came with the machine (TT-030). The >>one that was originally on there looked like it might have had a bit of >>glue over the join.... I can't remember if this is actually what happened. >>The manual gives no clarification either... >> >> >>Can you help??? >> >>thanks >> >>Karyne >>================================================================ >>Karyne Rogers tel +64 4 570 4636 >>GNS Science fax +64 4 570 4657 >>PO Box 31-312 >>Lower Hutt email [log in to unmask] >>New Zealand www.gns.cri.nz/stable >>================================================================