> Can someone advice me or suggest a reference on how much cabonate > sample (mollusks) is needed for isotope analysis of N, C, S and O on > Thermoelectron Delta V plus (Kiel IV). How about for elemental > analysis on TC/EA. My samples are pure CaCO3 (mollusk shells). Is > there a way to calculate the amount of sample needed based on the > percent of C in the sample? Any reference? I always tell the students: When you can see it we can measure it. The sample amount will range somewhere between 200 and 400 mikrogramms (10-6 gramm), but can be lower in special cases, depending on your setup. For a carbonate setup with the gasbench (not Kiel device) you may refer to: Spoetl C., and Vennemann T.W., 2003. Continuous-flow IRMS analysis of carbonate minerals. Rapid Communications in Mass Spectrometry, V. 17, p. 1004-1006. > The same question for water samples how much samples do I need for O > and H isotope analysis on Thermoelectron Delta V plus. Depends on the device on your massepec. Using an TC/EA will need only mikroliters while an equilibration unit will need a few milliliter. regards, Robert -- Dr. Robert van Geldern Leibniz Institute for Applied Geosciences (GGA) S3: Geochronology and Isotopehydrology Stilleweg 2, 30655 Hannover, Germany phone: +49-(0)511-643-2313 (office), -2539 (lab) fax: +49-(0)511-643-3665 mailto:[log in to unmask]