Jaime, We have been collecting waters from insects using an extraction line and did notice a significant effect on H and O isotope ratios from the volatile organics. We found that even a small amount of activated charcoal into the collection tube you can completely remove the VOCs from the extracted water and there is no fractionation associated with the charcoal. I'll send you some data off the list to demonstrate this. Presumably the situation will be similar with plant materials. Anthony ------------------------------------------------------ Anthony L. Michaud W. M. Keck Foundation Laboratory for Environmental Biogeochemistry School of Earth and Space Exploration Arizona State University Tempe, AZ 85287-1404 Web: http://kfleb.asu.edu Tel: 480-727-8033 Fax: 480-965-8102 Mailing address: Anthony Michaud PSF-686 Arizona State University Tempe, AZ 85287-1404 ***Windsurf Everywhere*** ------------------------------------------------------ -----Original Message----- From: Stable Isotope Geochemistry [mailto:[log in to unmask]] On Behalf Of Jaime Sent: Thursday, November 09, 2006 9:58 AM To: [log in to unmask] Subject: [ISOGEOCHEM] H-isotope Question I am currently conducting experiments analyzing H-isotopes in water from various plant materials. The water I have collected from the conifers is cloudy, so I am guessing that I also co-collected some small organic compounds. My method for analysis is pyrolysis at a high temperature (1200-1400 degrees C). I do not know how much of an effect the H-contribution from these compounds will be isotopically. I have enough volume to split the samples, and am thinking about adding activated charcoal to half the sample and then filtering it before running (after West et al. 2006). I am concerned about the potential for fractionation during this procedure. Has anyone else run into this problem, or have other suggestions? Thank you in advance. Best, Jaime L Toney