Kathy, We use UHP. The reference gas cylinders last an incredibly long time; so it isn't really that expensive in the long run. -- Charlotte Lehmann Assistant in Instruction Bates College Department of Geology 206A Carnegie Science Building 44 Campus Avenue Lewiston, ME 04240 Phone: 207-786-6485 FAX: 207-786-8334 Quoting Kathryn Venz Curtis <[log in to unmask]>: > Hi All, > > We are getting ready to measure the nitrogen isotopic values of N2O > produced via the "Bug Method" (i.e., Sigman et al. and Casciotti et > al.). I am looking into getting a N2O tank from our vendor to use as > the reference gas. What is the minimum grade N2O that is required for > use as the reference gas? Do I need UHP or can I get away with > something less expensive? There seems to be a variety of options at a > great range of cost. > > Thank you, > > Kathy > > > > -- > Kathryn Venz Curtis, PhD > Stable Isotope Mass Spec Lab Manager, Senior Chemist > Soil and Water Science Department > 2169 McCarty-A University of Florida > Gainesville, FL 32611-0292 Phone: (352) 392-1951 ext.242