Hi, Simon. 
We need to guess riddle: Why the Isodat is awaiting AS signal (as you wrote) , if there is not AS in the configuration? Unfortunately, I am not close to Trace GC. 
Vladimir. 

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На 14 мая 2018 г., 20:54, в 20:54, "Harlow, Benjamin" <[log in to unmask]> написал:п>Hi Simon, we also have a com cable labeled “computer” which runs from
>the GC to the computer. You did not describe one of these, which is new
>to me. Perhaps it just got unplugged?
>
>We do not have a second detector like you, but I looked up my
>handshaking to see it would be of help.
>
>Remote start: High to low
>GC ready out, inhibit ready, prep run out: when low
>End run out, start of run out: high to low
>
>Hope you get it resolved.
>
>Cheers, Ben
>
>From: Stable Isotope Geochemistry <[log in to unmask]> On Behalf
>Of Simon
>Sent: Sunday, May 13, 2018 3:31 AM
>To: [log in to unmask]
>Subject: Re: [ISOGEOCHEM] Aw: Re: [ISOGEOCHEM] "GC injected" signal not
>received by isodat
>
>Hey Vladimir!
>I already tried it with an old isodat backup, but did not solve my
>problem! :-(
>Thanks a lot for your continuous input!!
>
>Best,
>Simon
>
>
>On 12.05.2018, at 16:37, Vladimir
><[log in to unmask]<mailto:[log in to unmask]>> wrote:
>Hi, Simon. Did you create Backup Isodat in Version Handler when all
>system worked nice? If yes, do Restore. If not try to use Window
>restore point.
>Regards, Vladimir.
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>На 11 мая 2018 г., в 11:46, simon leitner
><[log in to unmask]<mailto:[log in to unmask]>> написал:п
>Dear Vladimir!
>
>Today I made a new configuration without any AS. I started a new
>sequence with a new IRMS method (isodat aquisition start: by device). I
>then manually started the GC, but isodat stayed at "waiting for AS
>trigger". hmm??
>
>best,
>simon
>
>Gesendet: Mittwoch, 09. Mai 2018 um 12:36 Uhr
>Von: Vladimir <[log in to unmask]<mailto:[log in to unmask]>>
>An: [log in to unmask]<mailto:[log in to unmask]>
>Betreff: Re: [ISOGEOCHEM] "GC injected" signal not received by isodat
>Hello Simon,
>But now do the configuration without of any AS, but with GC and see the
>start Acquisition after click in the Start button on GC. If all will be
>OK, do the new Isodat configuration with AS and new method and
>Sequence.
>Regards, Vladimir.
>
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>На 8 мая 2018 г., в 14:23, Simon <
>[log in to unmask]<mailto:[log in to unmask]>> написал:п
>Dear Vladimir!
>
>It is the same for both autosamplers!
>
>Simon
>
>On 07.05.2018, at 23:42, Vladimir <
>[log in to unmask]<mailto:[log in to unmask]>> wrote:
>
>Hello Simon,
>You wrote:
>... but after the autosampler injected a sample and the GC switches
>from "ready to inject" to "initial temp" isodat is not starting to
>measure
>Which Autosampler You mean  here? GC PAL?
>Vladimir.
>
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>На 7 мая 2018 г., в 15:12, Simon Leitner <
>[log in to unmask]<mailto:[log in to unmask]>> написал:п
>
>Dear Heavy Users!
>
>
>
>We have a Thermo Trace GC Ultra connected to a Delta V Advantage via GC
>Isolink and Conflo IV. The GC is equipped with two different
>autosamplers (VSP purge&trap, combi pal). A data transfer switch is
>used to change the communication from/to the GC with either one of the
>two samplers. The data aquisition is controlled/started via isodat 3.0.
>The system has been running since some weeks ago when I was changing
>from on-column injection by the VSP to PTV splittless injection by the
>combi pal (then i was also working on some errors with the PTV...)
>
>We now have the problem that isodat is not starting a "by device" run
>within a sequence. Isodat is as usually "waiting for AS trigger", but
>after the autosampler injected a sample and the GC switches from "ready
>to inject" to "initial temp" isodat is not starting to measure,
>executing the IRMS method respectively. While the previous steps of
>starting a run, waiting for "GC ready" and starting the injection by
>the autosampler is executed.
>
>Since I was klicking through the isodat configurator unaware that I
>maybe changed something I loaded an isodat backup, but our problem
>stayed the same.
>
>I should maybe add how the individual devices are connected to each
>other. The Isodat PC is connected via fiberline to the IRMS and the GC
>Isolink and by a COM cable to the GC. The GC is connected to the
>autosamplers via the "autosampler signals" plug-in and to the GC
>isolink and a second detector (Thermo ISQ) via the "generic handshake"
>plug-in (Y-cable). The connections are as they used to be when the
>measurement setup was running properly.
>
>Does anyone has a clue why the GC start signal is not received by
>isodat or how I could check if the GC signal is sent to isodat. Or is
>there any advise what to do in general?
>
>Thanks a lot!
>
>
>
>simon