Hi Greg
AOAC 998.12 is the usual method used to being honey C4 adulteration test only for EA-IRMS.
Protein precipitation protocol from honey is described in the method.
Cheers,
NJ
Dr. Nai-Jung, Wan
萬乃容
H&N- Health & Nutrition健康暨營養事業群
3F,No. 125, Wu Kung Road, Wuku Industrial Zone, New Taipei City, Taiwan 24886
24886
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From: Stable Isotope Geochemistry <[log in to unmask]>
On Behalf Of Christian Alexander Schopke
Sent: Thursday, May 20, 2021 2:43 AM
To: [log in to unmask]
Subject: [EXTERNAL] Re: [ISOGEOCHEM] EA-Honey analysis
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Hi Greg,
There are several articles related to the analysis of both bulk and compound-specific (e.g. protein fraction) IRMS of honey – see for example the following review articles that come
at the issue quite broadly:
Anklam (1998) A review of the analytical methods to determine the geographical and botanical origin of honey. Food chemistry 63 549-562
Siddiqui et al (2017) Application of analytical methods in authentication and adulteration of honey. Food chemistry 217 687-698
There are also several isotope-specific papers about honey, for example (and there are many others):
Kropf et al (2010) Carbon and nitrogen natural stable isotopes in Slovene honey: adulteration and botanical and geographical aspects. J. Agric. Food Chem. 58, 24, 12794-12803
A few years ago I bought 4-5 different honeys and analyzed them for C/N via EA-IRMS just for fun and did not observe any significant differences, I never got as far as extracting the
protein though.
Hope this helps,
Christian
Christian Schöpke
Senior Engineer
Institute for Energy Technology
Instituttveien 18, 2007 Kjeller, NORWAY
From: Stable Isotope Geochemistry <[log in to unmask]>
On Behalf Of Cane, Gregory A
Sent: onsdag 19. mai 2021 17:50
To: [log in to unmask]
Subject: [ISOGEOCHEM] EA-Honey analysis
Has anyone ever tried analyzing honey via EA-IRMS? Is this at all advisable?
Regards,
Greg