We regularly run honey, maple syrup, and corn syrup samples as part of our
fourth year undergraduate course at McMaster University. It's a fun lab for
students and clear differences can be seen between natural honey and
adulterated products. We have only used the bulk honey and have never
extracted the protein, although we do discuss this in lab.

We typically weigh the honey on tin foil discs and bundle the sample up. I
find using stable isotopes to identify the adulteration and woukd be happy
to discuss this in further detail with you.

On Wed, May 19, 2021, 7:12 PM Joel Avrunin, <
[log in to unmask]> wrote:

> Gregory,
>
>
>
> The FDA in Cincinnati wrote a poster comparing EA-IRMS to the Picarro CRDS
> for detection of honey adulteration. There is a bit here on method
> development as well:
>
>
> https://www.picarro.com/support/literature/a_comparison_of_irms_and_crds_for_the_detection_of_economic_adulteration_of
>
>
>
> We also have a few app notes on using an EA with a CRDS for honey
> adulteration:
>
>
> https://www.picarro.com/support/library/documents/an022_combustion_modulecrds_for_d13c_analysis_of_imported_honey_and
>
>
> https://www.picarro.com/support/library/documents/picarros_combustion_modulecrds_provides_excellent_data_using_the_approved
>
>
>
> One limitation on method – this will work for C4 adulterated samples such
> as those adulterated with Corn Syrup, but unfortunately won’t effective
> with those using sugars such as rice syrup.
>
>
>
> *Joel Avrunin*
>
> Email  [log in to unmask]
>
> Twitter https://twitter.com/JoelAtPicarro
>
>
>
> *From:* Stable Isotope Geochemistry <[log in to unmask]> *On Behalf
> Of *Howe, Stephen S
> *Sent:* Wednesday, May 19, 2021 5:23 PM
> *To:* [log in to unmask]
> *Subject:* Re: [ISOGEOCHEM] EA-Honey analysis
>
>
>
> Greg,
>
>
>
> We analyzed a number of samples of honey and maple syrup using EA-IRMS to
> convincingly demonstrate the effect the addition of corn sweeteners had on
> the d13C values of unadulterated products at the University at Albany many
> years ago.  We never had any issues with viscous liquids such as these
> dribbling out of the tin cups.  If you run lots of samples, you’ll want to
> keep an eye on your water trap.
>
>
>
> Steve
>
>
>
>
>
> On May 19, 2021, at 11:50 AM, Cane, Gregory A <[log in to unmask]> wrote:
>
>
>
> Has anyone ever tried analyzing honey via EA-IRMS? Is this at all
> advisable?
>
>
>
> Regards,
>
>
>
> Greg
>
>
>