>> Hi, Everybody: When I use hydrochloric gas to acidify high pH water >>samples for oxygen isotope ratio analysis using CO2-H2O equilibration >>method, I found the Delta O-18 of the water shifts to lighter values as >>the pH goes lower. Any explanation? Dachun Zhang Dear Dachun, Maybe the O isotope fractionation between H3O+ and H2O is responsible? Looking in Friedman and O'Neil (1977), Table 4, #8, the O isotope fractionation between H3O+ and H2O is given as +22.8 per mil [actually the reaction is given as H2O+1 - H2O(l), but I assume this should be H3O+. The original reference, Thornton (1962), is given]. If you are acidifying to sufficiently low pH, the concentration of H3O+ may be high enough to affect your measured delta18O values. Moreover, it will shift them to lighter values (as you observe), as H3O+ is isotopically heavy vs. H2O(l). I hope this helps. Cheers, Simon ***** Simon R. Poulson Dept. of Geology & Geophysics University of Wyoming Laramie, WY 82071-3006 USA (307) 766-2885 Fax: (307) 766-6679 [log in to unmask] *****