Dear Lars, From the comments by Pier de Groot and Andrew Tait, you might want to consult the following references. Krishnamurthy, R. V., Atekwana E. A., and Hillol Guha, 1997. A simple, inexpensive carbonate-phosphoric acid reaction method for the determination of the ?13C and ?18O of carbonates. Analytical Chemistry, 69, 4256, 4258 Swart, P. K Burns, S. J. and Leder, J.J. 1991. Fractionation of the stable isotopes of oxygen and carbon in the carbon dioxide during the reaction of calcite with phosphoric acid as a function of temperature and technique, Chemical Geology, 86, 89-96 Al-Aasm, I H., Taylor, B. E., and South, B. 1990. Stable isotopic analysis of multiple carbonate samples using selective acid extraction. Chemical Geology, 80,119-125. Eliot Atekwana Assistant Professor Department of Geology (317) 274 7969 (office) IUPUI (317) 274 7484 (dept.) 723 W Michigan Street (317) 274 7966 (fax) Indianapolis IN 46202-5132 e-mail: [log in to unmask] www.geology.iupui.edu Dear listmember I was asked to determine carbonate content in a solid powder and did the following with my VG Optima GC-IRMS normally in use for simple breath analysis. Is this a good method? Improvements suggested? * 100mg solid powder put in 1.8 ml gas tight glass vials * Vials flushed with pure helium in a glove chamber to reduce background CO2 * 200 ul 0.1M phosphouric acid (>10x molar excess) added with a syringe via the septa * mix 5 min wait 30 minutes * Headspace gc-irms to get total CO2 and isotope ratios as a bonus Got nice results anyway, but have to be sure I did not do any major methodology or practical mistakes Thanks! By the way - would it be possible to tell what carbonate was in the material from the delta values? Got -3.4 for my unknown samples and -8.0 from my Na2CO3 standard. Does it tell me anything else then that it was not laboratory air CO2 in my samples? Best wishes, Lars