Dear Chris There is a method for DIC analysis that does not need preservation of the sample, is simple and reliable. The reference is given below. I am sure others can address the issue of S-34 and sample preservation. Atekwana E. A. and Krishnamurthy, R. V., 1998. Seasonal variations of dissolved inorganic carbon and ?13C of surface waters: Application of a modified gas evolution technique. Journal of Hydrology 205, 265-278. Good luck Eliot Atekwana Assistant Professor Department of Geology (317) 274 7969 (office) IUPUI (317) 274 7484 (dept.) 723 W Michigan Street (317) 274 7966 (fax) Indianapolis IN 46202-5132 e-mail: [log in to unmask] www.geology.iupui.edu -----Original Message----- From: Christopher Romanek [SMTP:[log in to unmask]] Sent: Thursday, October 07, 1999 7:25 AM To: [log in to unmask] Subject: DIC analysis Dear subscribers, I have a student who is collecting water samples for dissovled inorganic carbon (C-13) and sulfide (S-34) analysis (both at 100's of ppm). I usually add a few granules of solid HgCl to vials to inhibit microbial activity for samples lacking sulfide, but I'm worried about sequestering Hg as HgS, potentially compromising both C-13 and S-34 analyses. Can anyone please offer advice or a protocol for such samples (different way to "poison" samples)? I apologize in advance if this issue has been broached recently. Chris Romanek Savannah RIver Ecology Lab