I have been running FAMEs on our GC-c-IRMS
using a 30m DB-5 column with 5m of guard column. The first six samples
returned good peak resolution. After the sixth, I now get very poor resolution.
Instead of well-defined peaks at the expected retention times, I'm getting
hundreds of peaks at low amplitude (<100mV) throughout the run.
The GC method I'm currently using is as
90-280C, 3C/min., 20 min hold; Flow rate 1.2
ml/min. He; Injection: splitless w/ surge.
Can anyone tell me what's happened, and how it
might be fixed?
Melissa D. Bautista
Centre for Coastal Biogeochemistry
P.O. Box 157 Military Road
Lismore NSW 2480
Phone (02) 66