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Hi Wolfram,

Thank you for replying. I have answered your questions as best I can below. Our GC is a Thermo Trace Ultra, The combustion interface is the Finnigan III, and the IRMS is the Delta V Plus.


1. What is the temperature setting for the injector? 260C
2. What's the average concentration per FAME in your FAME mix (in nmol/microL)? Unsure. Samples were extracted from both seagrass and mangrove sediments, but I only received them ready for methylation. I was told that the total lipid weight was between 1-2mg.
3. Have you looked what the chromatogram looks like for a dummy run (no injection or neat solvent only)? I have run hexane only with similar results (can send you the Isodat run file if that would help).
4. Same as point 3 but for single compound sample, say 10:0 or 12:0. I have not done this, but will do so now.
5. Is your system fitted with a FID and a cross-piece prior to the oxidation recator? Yes, FID and cross-piece.

King regards,

Melissa





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