Hi Anne We use double distilled dichloromethane (DCM) as the solvent of choice for removing lipids from tissue samples and extracting lipids for analysis from soils and plant material. We use a Dionex accelerated solvent extractor(ASE 200) run at 100oC and 2000psi - two 5-minute cycles recovers all the lipids. Cheers Max Max Gibbs NIWA, PO Box 11-115 Gate 10 Silverdale Rd Hamilton 3251 New Zealand Phone: +64 7856 1773 Fax: +64 7856 0151 Cell: 027 604 1449 Email: [log in to unmask] >>> Fname Lname <[log in to unmask]> 5/11/2009 7:06 a.m. >>> While looking at the scientific literature, there seems to be quite a spread of solvents used to extract lipids from tissues ultimately analyzing the tissue for 15N & 13C. New to this, I am trying to veer from the chloroform/methanol solution. Are there any other solvents that people are happy using generating accurate isotopic results? I ultimately plan on capturing the lipid fraction and analyzing 15N & 13C as well as the lipid extracted muscle tissue. Thanks, Anne ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Anne M. Cotter U.S. Environmental Protection Agency Mid-Continent Ecology Division 6201 Congdon Blvd Duluth, MN 55804 218-529-5183 fax 218-529-5003 [log in to unmask] ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ NIWA is the trading name of the National Institute of Water & Atmospheric Research Ltd.