Hi Million,
I guess it depends at a new focusing of the lenses. I think you did not 
change the focus settings. So you use the focusing with dirty lenses, 
but this is different to clean lenses.
You should do a magnet scan slow magnet first. If there are peaks, the 
next step is a new autofocus like Andrew has written.

Good luck,

Million Hailemichael schrieb:
> I removed and cleaned the ion source for our delta V advantage. I changed a 
> new filament and I put back the source and everything looks fine on isodat 
> screen. Values areā€¦
> HV= 3.01KV
> Box= 0.72 mA
> Trap= 0.77 mA
> vacuum= 1.4e-006
> Magnet (steps)= 11572 
> When I select reference gas (CO2) on the conflo screen to do a jump 
> calibration no CO2 gas is flowing into the system. I checked for a leak and NO 
> leak, we have a half tank CO2 gas. It seems ions are not reaching the cups or 
> ions are not generated. I checked the CO2 tank is open, conflo is open and 
> SGE valve is open.
> I am planning to remove the source again and see if the small opening in the 
> side of the ion source chamber is somehow blocked or miss aligned (even 
> though I checked every thing before I put it back) . Or when I pushed the 
> source into the mass spectrometer possibly a wire may have been 
> disconnected?. 
> Any advice or suggestions before I remove the ion source again to check for 
> any loose connection.
> Thanks
> Million

Sven Steinbrenner

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