While I cannot provide you with a tried and tested Uniprep method, I can point you to a couple of papers you could use (a) as a comparison wrt 2H abundance in cellulose and typical exchange fraction, and (b) as a source of reference materials.
The Dynamic Duo at USGS Reston (aka Haiping Qi and Tyler Coplen) have prepared powdered whole-wood standards, USGS54, USGS55, and USGS56. All three have been characterised for 2H, 13C, 15N, and 18O. Obviously, whole wood is not the same as pure cellulose but these three standards should serve well for the purpose of scale anchoring and quality control.
The corresponding paper by Qi, Coplen, and Jordan was published in Chemical Geology (2016), vol. 442, pp. 47-53. http://dx.doi.org/10.1016/j.
The hydrogen exchange fractions of 5.4, 4.1, and 6.6% Qi, Coplen, and Jordan reported for their whole wood materials was in very good agreement with the exchange fraction of 4.6% (theoretical = 5%) we determined for raw (unprocessed) cotton.
H.F. Kemp, E. R. Schenk, and J. R. Almirall (2014): “Discrimination of unprocessed cotton on the basis of geographic origin using multi-element stable isotope signatures”; Rapid Commun. Mass Spectrom., 28, 545-552; DOI: http://dx.doi.org/10.1002/rcm.
Prof. Dr W Meier-Augenstein, CChem, FRSC
Stable Isotope Forensics & Analytical Sciences
School of Pharmacy & Life Sciences
The Sir Ian Wood Building
Stable Isotope Forensics book, 2nd edition: https://www.wiley.com/en-gb/
Stable+Isotope+Forensics%3A+ Methods+and+Forensic+ Applications+of+Stable+ Isotope+Analysis%2C+2nd+ Edition-p-9781119080206
I'm trying to come up with a method to put our Uniprep autosampler to good use (if I manage to fix or ignore all those leaks...). I saw a few papers but I'm sure there is more out there. If you had a method that gives nice results (but above all, good reproducibility) that you wouldn't mind sharing, that would be great.
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