>Dear Isogeochem subscribers-
>I'm interested in extracting lipids from prehistoric (low-fired)
>order to analyze carbon isotopes. I'm looking for C4 vs. C3 plant
>in the ceramic matrix. I have found literature on lipid extraction
>Evershed, Heron and others, but have not found other sources. Have
>you done such work? If so, I would greatly appreciate any
>especially references outside strictly archaeological literature.
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>Penn State Univeristy
>University Park, PA 16802
The classic one with CHCl3-MeOH is
Bligh, E.G. Dyer, W.J.
A rapid method of total lipid extraction and
GenevaCan. J. Biochem.
Collister et al.
Organic Geochemistry 21, 645-659 (1994).
Schnitzer and Neyroud
Fuel 54, 17-19 (1975)
Lichtfouse et al. Organic Geochemistry 23, 865-868 (1995).
15 min sonication with distillated CH2Cl2 in a beaker (3 times) then
filtration on cellulose acetate filters (those of coffee machines give
excellent filtration) then concentration under vacuum. Glassware and
filters must be pre-washed with distillated CH2Cl2 (of course). For
very small samples, extration and filtration may be achieved at the
same time in about 5 min. on a pipette Pasteur loaded with coton wool,
sand and a bit of thin silica gel (Henner et al, Analusis 25, 51-52